ace2  (R&D Systems)

Journal: Cell Death Discovery

Article Title: Modelling severe COVID-19 in TLR3-mutated hiPSCs-derived lung organoids

doi: 10.1038/s41420-025-02936-5

Figure Lengend Snippet: A Phase–contrast microscopy of alveolospheres embedded in 3D Matrigel at day 51 of culture. Scale bar 200 µm. B Haematoxylin and eosin-stained hLORG cross-section showing the typical epithelial morphology. Scale bar 50 µm. C–E ACE2, SFTPC protein and gene expression in hLORGs at day 51 of differentiation by immunohistochemistry and qPCR analysis. F FOXA2 protein expression in hLORGs at day 51 of differentiation by immunohistochemistry. Scale bar 50 µm. G DPP4 protein expression in hLORGs at day 51 of differentiation by immunofluorescence. Scale bar 50 µm. H , I Immunofluorescence images show the overall actin (red) and α-tubulin distribution (green) of an hLORG displaying a prominent epithelial structure (nuclei, blue). Scale bar 50 µm and 25 µm, respectively.

Article Snippet: Immunohistochemistry was performed on the organoids fixed in 4% formalin (Spitalieri et al.), using goat anti-ACE2 antibody (1:60, AF 933, R & D Systems, Minneapolis, Minnesota, MN, USA) and rabbit anti-SFTPC (1:1000, ab 3786, Chemicon, Temecula, California, CA, USA).

Techniques: Microscopy, Staining, Gene Expression, Immunohistochemistry, Expressing, Immunofluorescence